BD OneFlow™
PCD Tube

Overview

The BD OneFlow™ PCD Tube is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.¹*

The BD OneFlow™ PCD Tube, when run in parallel with the BD OneFlow™ PCST, is intended for flow cytometric immunophenotyping of normal and aberrant plasma cells in bone marrow as an aid in the diagnosis of multiple myeloma or other plasma cell disorders.

Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ LST, BD OneFlow™ B-CLPD T1, BD OneFlow™ PCST, and BD OneFlow™ A LOT.

FEATURES

The BD OneFlow™ PCD Tube:

Is a preconfigured single-use, ready-to-use 8-color reagent
Is a classification tube that can be used for specimens with plasma cell populations needing further investigation as determined by the BD OneFlow™ PCST
Is available in the 10 test/box size (4 pouches of 5 tubes each)
Allows for easy visual identification with boxes, pouches and tubes color coded with a lighter green color than the one identifying the BD OneFlow™ PCST

The BD OneFlow™ Reagents increase efficiency by simplifying instrument standardization, reducing the time to prepare the instrument as well as the technical burden and training needs.²

For BD FACSLyric™ Flow Cytometer users:

BD^® CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility through Universal Setup
BD^® FC Beads (7-Color, 5-Color and 2-Color Kits) support consistency of results, eliminating the need for using cells for compensation
BD^® CS&T Beads and BD^®FC Beads kits necessary for instrument setup and compensation can be used for other CE-IVD assays on the BD FACSLyric™ Flow Cytometer

For BD FACSCanto™ II Flow Cytometer users:

BD FACSDiva™ CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility with Application Settings
BD OneFlow™ Setup Beads ensure data accuracy and reproducibility by providing assay-specific target values, as per EuroFlow™ SOPs³
Specifically designed BD^® FC Beads eliminate the need for using single-vial reagents and cells for compensation

The BD OneFlow™ PCD Tube reagent composition

Antibody	Fluorochrome	Clone	Target Populations
CD38	FITC	HB7	Backbone marker. Identification of plasma cells
CD28	PE	L293	Identification of aberrant plasma cell populations
CD27	PerCP-Cy 5.5	L128	Identificaiton of aberrant plasma cell populations
CD19	PE-Cy 7	SJ25-C1	Backbone marker. Identification of plasma cells
CD117	APC	104D2	Identificaiton of aberrant plasma cell populations
CD81	APC-H7	JS81	Identificaiton of aberrant plasma cell populations
CD45	BD Horizon V450	2D1	Backbone marker. Identification of plasma cells
CD138	BD Horizon™ 500-C	MI15	Backbone marker. Identification of plasma cells

The EuroFlow antibody panel article¹* has a full description of the utility of the antibodies chosen for the BD OneFlow™ PCD Tube.

APPLICATIONS

BD OneFlow™ LST Lab Report (normal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of a normal bone marrow specimen stained with the 8-color 12-antibody BD OneFlow™ LST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages.

BD OneFlow™ LST Lab Report (abnormal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of an abnormal bone marrow specimen stained with the 8-color 12-antibody BD OneFlow™ LST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages.

BD OneFlow™ Reagents can be run on both the BD FACSLyric™ and BD FACSCanto™ II Flow Cytometers with equivalent results.

Supporting Evidence

The EuroFlow “PCD panel was able to detect and fully characterize the immunophenotype of aberrant plasma cells or their normal/reactive counterparts in all the samples tested (100), as also assessed by routine diagnostic protocols performed in parallel.”¹*

RESOURCES

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Instructions for Use

BD OneFlow™ Setup Beads (BD FACSCanto™ II Flow Cytometer)

BD^® FC Beads 8-Color Kit for BD OneFlow™ Assays (BD FACSCanto™ II Flow Cytometer)

Tools

BD Antibodies in EuroFlow™ Multicolor Panels

EuroFlow™ Multicolor Panels

Related Resources

Application Note

Standardized Flow Cytometry System to Aid in the Diagnosis of Chronic Lymphocytic Leukemia

References

van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122

BD OneFlow™ PCD Tube