Built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis,1* the innovative BD OneFlow™ Solution brings the standardization of leukemia and lymphoma immunophenotyping one step forward, improving laboratory efficiency and enabling reliability and accuracy of results for clinical decisions and ultimately patient outcome.2, 3 The BD OneFlow™ Solution is a comprehensive set of reagents (LST, B-CLPD T1, PCST, PCD and ALOT), setup beads, protocols, and assay acquisition and analysis templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations.
BD OneFlow™ LST
The BD OneFlow™ LST is a preconfigured, ready-to-use 8-color 12-antibodies reagent that is provided in a single-test tube format. It is intended for flow cytometric immunophenotyping of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages in peripheral blood, bone marrow and lymph nodes.
BD OneFlow™ B-CLPD T1
The BD OneFlow™ B-CLPD T1 is a preconfigured, ready-to-use 8-color reagent provided in a single-test tube format. It shall be used for specimens with B cell lineage populations needing further investigation in combination with the BD OneFlow™ LST. B-CLPD T1 is intended for flow cytometric immunophenotyping of B cells in peripheral blood and bone marrow.
BD OneFlow™ PCST
The BD OneFlow™ PCST is a preconfigured, ready-to-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube). It is intended for flow cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow.
BD OneFlow™ PCD
The BD OneFlow™ PCD Tube is a preconfigured, ready-to-use 8-color reagent provided in a single-test tube format. When run in parallel with the BD OneFlow™ PCST, it is intended for flow cytometric immunophenotyping of normal and aberrant plasma cells in bone marrow.
BD OneFlow™ ALOT
The BD OneFlow™ ALOT is a preconfigured, ready-to-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube). The tube is intended for flow cytometric immunophenotyping of aberrant immature populations of hematopoietic cells (lymphoid and non-lymphoid lineage) in bone marrow and peripheral blood.
Why BD OneFlow™ Solution?
- Efficient workflows to reduce manual workload
- Ready-to-use, single-test tube format for direct specimen staining
- Convenient dried format
- Comprehensive solution of reagents, beads, protocols and assay templates
Our Leadership
Our innovative BD OneFlow™ Solution brings the standardization of leukemia and lymphoma immunophenotyping one step forward.
References
1. van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
2. van der Velden VHJ, Flores-Montero J, Perez-Andres M, et al. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2019;475:112287. doi: 10.1016/j.jim.2017.03.011
3. Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
