For Professionals in Research
Overview
The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit enables you to generate highly reproducible WTA data on the BD Rhapsody™ Single-Cell Analysis System. The kit has been tested and validated to generate consistent, high-quality whole transcriptome data across different workflows and users and a wide range of cell inputs.
Learn more from the BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit Brochure.
FEATURES
The BD Rhapsody™ WTA Amplification Kit allows you to build targeted panels based on your WTA data
The kit:
Allows you to identify the most differentially expressed genes in your samples, which can then be used to construct a targeted panel specific to your research needs
Is designed to work reliably across a wide range of cell inputs from 1,000 to 10,000 cells
Works seamlessly with the BD® Single-Cell Multiplexing Kit and enables you to run multiple samples at once without compromising the quality of your data and to save time and money
PERFORMANCE
The BD Rhapsody™ WTA Amplification Kit generates consistent and reproducible data across multiple users and cell types
Reproducible data from the same sample processed using two different cartridges
Reproducible WTA data from the same sample processed by three different users
Consistent data across a wide range of cell inputs
The BD Rhapsody™ WTA Amplification Kit has been used to identify major immune cell types
t-SNE plot showing the major immune cell types identified with the BD Rhapsody™ WTA Amplification Kit.
The BD Rhapsody™ WTA Amplification Kit can be used in combination with the BD® Single-Cell Multiplexing Kit to analyze multiple samples simultaneously
Jurkat and Ramos cells labeled with four unique sample tags from the BD® Human Single-Cell Multiplexing Kit were mixed at an equal ratio (1:1:1:1), pooled and processed using the BD Rhapsody™ Single-Cell Analysis System. Libraries were generated using the BD Rhapsody™ WTA Amplification Kit.
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Brochure
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Protocols and Handbooks
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BD® Single-Cell Multiomics Analysis Setup User Guide
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BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
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BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol
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BD Single-Cell Genomics Bioinformatics Handbook
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BD Rhapsody™ WTA Amplification Kit Product Insert
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Multiomics Protocols (WTA + AbSeq)
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
